Article | . 2017 Vol. 35, Issue. 1
Several Factors Affecting Mass Production of Microlepia strigosa (Thunb.) C. Presl Sporophytes

Department of Horticultural Science, Chungbuk National University,1

2017.. 46:58


This study was conducted to investigate the optimal conditions for spore germination, prothallus propagation, sporophyte formation and seedling growth in Microlepia strigosa (Thunb.) C. Presl. Spore germination and prothallus development were promoted by low concentrations of Knop medium nutrient solution. The optimal medium for prothallus propagation and antheridium formation was 2X MS medium with 3% sucrose. The activated charcoal content of the medium did not affect prothallus proliferation. Among the various combinations of culture soil (bedding soil, peat moss, perlite and decomposed granite), a mixture of bedding soil, peat moss and decomposed granite at a ratio of 1 : 1 : 1 (v : v : v) had a positive effect on sporophyte formation. The most efficient conditions for promoting the growth of whole plants (sporophyte seedlings) were 50 - cell plug trays filled with a mixture of bedding soil and decomposed granite at a 2 : 1 (v : v) ratio.

1. Ambrosio ST, de Melo NF (2004) Interaction between sucrose and pH during culture. Acta bot bras 18:809-813. doi:10.1590/S0102-33062004000400011  

2. Argo WR (1998) Root medium physical properties. HortTechnology 8:481-485   

3. Bold HC, Alexopoulos CJ, Delevoryas P (1987) Morphology of plant and fungi. Harper Collins Publishers, NY, USA  

4. Chang HC, Agrawal DC, Kuo CL, Wen JL, Chen CC, Tsay HS (2007) culture of Drynaria fortunei , a fern species source of Chinese medicine “Gu-Sui-Bu”. Cell Dev Biol-Plant 43:133-139. doi:10.1007/s11627-007-9037-6   

5. Choi JM, Ahn JW, Ku JH, Lee YB (1997) Effect of medium composition on physical properties of soil and seedling growth of redpepper in plug system. J Korean Soc Hort Sci 38:618-624  

6. Choi JM, Park JM, Latigui A (2011) Impact of physicochemical properties of root substrates on growth of mother plants and occurrence of daughter plants in ‘Seolhyang’ strawberry propagation through bag culture. Korean J Hortic Sci Tecnol 29:95-101   

7. Cox J, Bhatia P, Ashwath N (2003) spore germination of the fern . Sci Hort 97:369-378. doi:10.1016/S0304-4238(02)00152-8  

8. Fernandez H, Bertrand AM, Feido I, Sanchez-Tames R (1997) Gametophyte culture and antheridiogen activity in . Plant Cell Rep 16:358-362  

9. Fernandez H, Revilla MA (2003) culture of ornamental ferns. Plant Cell Tiss Org Cult 73:1-13. doi:10.1023/A:1022650701341  

10. Jang HR, HJ Lee, AM Shohael, BJ Park, KY Paek, SY Park (2016) Production of biomass and bioactive compounds from shoot cultures of Rosa rugosa using a bioreactor culture system. Hortic Environ Biotechnol 57:79-87  

11. Jeong JA, Lee CH. (2006) Prothallus morphogenesis of (L.) Presl and var. Nakai culture. Kor J Plant Res 19:360-364  

12. Knop W (1865) Quantitative untersuchungen uber die ernahrungsprozesse der pflanzen. Landwirtsch Vers Stn 7:93-107  

13. Korea Biodiversity Information System (KBIS) (2015) Korea forest service.  

14. Lee JS (2001) Several factors affecting sporophyte formation of three species in Pteridophyta. MS Thesis, Chungbuk National University, Cheongju, Korea  

15. Ministry of Environment (ME) (2007) 3rd. Natural environment survey in flora. Natl Inst Environ Res, Incheon, Korea  

16. Moshkov IE, Novikova GV, Hall MA, George EF (2008) Plant growth regulators III. In George EF, Hall MA, de Klerk GJ eds, Plant propagation by tissue culture, 3rd. Springer, Netherland  

17. Nelson PV (2003) Greenhouse operation and management. 6th ed, Prentice Hall, Englewood Cliffs, NJ, USA  

18. Park JA, BJ Park, AH Kim, SY Park, KY Paek (2015) Airlift bioreactor system and nitrogen sources for biomass and antioxidant compound production from culture of plantlets. Hortic Environ Biotechnol 56:358-365  

19. Shin BK, Son JE, Choi JM (2012) Physico·chemical properties of peatmoss and coir dust currently used as root medium components for crop production in Korean plant factories. J Bio-Environ Cont 21:362-371. doi:10.12791/KSBEC.2012.21.4.362   

20. Shin SL, Lee CH (2009) medium composition and culture method affecting masspropagation of Thunb.Prothalli. Korean J Hortic Sci Technol 27:299-304  

21. Shin SL, Lee MY, Choi JS, Lee CH (2009) Effect of medium components and culture methods on prothallus propagation of aquilinum var. (Desv.) Underw. ex Hell. Kor J Plant Res 22:337-342  

22. Shin SL, Lee MY, Choi JS, Lee CH (2010) Effect of plant growth regulators on sporophyte formation from cultured prothallusn of bracken fern. Korean J Hortic Sci Technol 28:301-306  

23. Shorina NI (2001) Population biology of gametophytes in homosporous Polypodiophyta. Russ J Ecol 32:164-169.doi:10.1023/A:1011306010154  

24. Toole EH, Tottingham WE (1918) The influence of certain added solids upon the composition and efficiency of knop's nutrient solution. Am J Bot 5:452-461. doi:10.2307/2435152  

25. Wang, Y, BL Dunn, DB Arnall (2012) Assessing nitrogen status in potted geranium through discriminant analysis of ground-based spectral reflectance data. HortScience 47:343-348  

26. Warncke PD (1986) Analysing greenhouse growth media by the saturation extraction method. HortScience 211:223-225  

27. Whittier DP (1975) The influence of osmotic conditions on induced apogamy in gametophytes. Phytomorphol 25:246-249