Article | . 2018 Vol. 36, Issue. 3
Efficiency of Vegetable Soft Rot Pathogen Pectobacterium carotovorum subsp. carotovorum(Pcc21) as a Host Cell for Foreign Gene Expression

Department of Horticulture and Life Science, Yeungnam University1

2018.. 426:434


Enhanced introduction of foreign genes into plants has been achieved through the development of expression vectors and the use of diverse host cells. Escherichia cili and Agrobacterium tumefaciens are commonly used for plant transformation. However, there is still high demand for development of new host systems for foreign protein production in biotechnology and medical industry. Recently Pectobacterium carotovorum subsp. carotovorum (Pcc21), a pathogen causing soft  rot disease in potato, has received attention for biotechnological use because of its ease to grow and transform. In this study, we examined the potential of P. carotovorum (Pcc21) as a novel host system by assessing the efficiency of GFP protein production compared with other common host systems E. coli (DH5α) and A. tume-faciens (LBA4404). GFP production was evaluated by both RT-PCR and immunoblotting. Pcc21 produced about 30% more GFP protein than did DH5α and LBA4404. We suggest that Pcc21 is a potent candidate host to be used for foreign protein production as well as to be used as substitute for A. tumefaciens for transient and/or stable transformation of plants due to its high efficiency in in-fecting plants.

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