Article | . 2018 Vol. 36, Issue. 4
A Phylogenic Analysis of Citrus Cultivars Native to Jeju using Chloroplast DNA trnL-trnF and Internal Transcribed Spacer Region Sequences



Citrus Research Institute, National Institute of Horticultural and Herbal Science1
R&D Coordination Division, Rural Development Administration2
Planning and Coordination Division, National Institute of Horticultural and Herbal Science3




2018.. 585:597


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This study was carried out to determine the phylogenetic relationships among 28 Citrus species, including 11 cultivars native to Jeju, Korea, based on the sequences of the trnL-trnF region in chloroplast DNA and the internal transcribed spacer (ITS) region in nuclear ribosomal DNA. The average total length of the trnL-F region in chloroplast DNA was 361.1 bp, and that of the conserved sequence site was 354 bp, that of the variable site was 7 bp, and that of the parsimonious informative site was 2 bp. The average total length of the ITS region, which contains the 5.8S rDNA region, was 637.68 bp, and that of the conserved sequence site was 570 bp, that of the variable site was 68 bp, and that of the parsimonious informative site was 25 bp. Analysis of sequence divergence and phylogenetic relationships among the citrus species revealed that most native citrus cultivars were closely related, and these cultivars were presumed to be obtained by cross-breeding with pomelo (Citrus maxima) and mandarin (C. reticulata). In particular, the ‘Byungkyool’ cultivars, presumed to be a unique cultivar genetically and morphologically selected on Jeju island, was confirmed to have been obtained by long-term hybridization with cultivars of pomelo (‘Dangyooja’, etc.) and mandarin (‘Hongkyool’, etc.). However, analysis of the phylogenetic relationships among citrus cultivars native to Jeju was difficult due to the propagation through seedling from unknown cross combinations long time ago. These results suggest that phylogenetic analysis could be used to generate basic data about native citrus cultivars on Jeju island.



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