Article | . 2018 Vol. 36, Issue. 4
Characterization of Hydrangea Accessions Based on Morphological and Molecular Markers

Department of Horticulture, Chonnam National University1
Department of Biotechnology, Konkuk University3

2018.. 598:605


Hydrangea (Hydrangea spp.) is commonly cultivated as an ornamental plant for its attractive characteristics. While numerous hydrangea cultivars are available as genetic resources, they cannot be utilized effectively for breeding purposes because of a lack of information on their genetic relationship and breeding compatibility. In this study, genetic relationships within a collection of 34 accessions consisting of 4 species of hydrangea (H. macrophylla, H. paniculata, H. serrata, and H. arborescens) were evaluated using random amplified polymorphic DNA (RAPD) markers. The hydrangea cultivars were maintained in the greenhouse at Chonnam National University. The dendogram of the genetic relationship was subjected to a clustering analysis in the PAST program using the unweighted pair group method with arithmetic mean (UPGMA) method to estimate the genetic distances and relatedness among the species. Based on RAPD analysis, H. macrophylla was separated into two major groups with high levels of genetic similarity among the cultivars. H. paniculata accessions were classified into one major group with ‘Womseuredeu’ genetically distant from the other cultivars. H. serrata and H. arborescens were divided into two separated groups. The dendrogram suggests that H. macrophylla and H. serrata are genetically similar therefore the chance of hybridization between this species may be greater than that between more distantly related species. The present study provides useful information for the breeding of hydrangea cultivars.

1. Dirr MA (2002) In search of a perfect Hydrangea. Nursery Mgt Production 18:16-17, 95-96  

2. Dirr MA (2004) Hydrangeas for American Gardens. Portland, OR: Timber Press   

3. Haworth-Booth M (1984) The hydrangeas, 5th ed. Constable, London, UK   

4. Jaccard P (1901) Étude comparative de la distribution floraledansune portion des Alpes et des Jura. Bulletin de la Societe Vaudoise des Sciences Naturelles 37:547-579. doi:10.5169/seals-266450  

5. Joung YH, Roh MS (2010) Identification of Hydrangeaceae accessions of wild origin from Jeju, Korea, using molecular markers. Plant Genet Resour: 8:235-241. doi:10.1017/S1479262110000286  

6. Jukes TH, Cantor CR (1969) Evolution of protein molecules. In HN Munro, ed, Mammalian Protein Metabolism. Academic Press, New York, USA, pp 21-132. doi:10.1016/B978-1-4832-3211-9.50009-7  

7. Kim DK, Seo SG, Kwon SB, Park YD (2016). Development of RAPD and SCAR Markers related to watermelon mosaic virus and zucchini yellow mosaic virus resistance in Cucurbita moschata. Hortic Environ Biotechnol 57:61-68. doi:10.1007/S13580-016-0090-0  

8. KudoN, Matsui T, Okada T (2008) A novel interspecific hybrid plant between Hydrangea scandens ssp. Chinensis and H. macrophylla via ovule culture. Plant Biotechnol 25:529-533. doi:10.5511/plant biotechnology.25.529  

9. Makino T (1929) A contribution to the knowledge of the flora of Japan. J Jap Bot 6:11-12   

10. McClintock E (1957) A monograph of the genus Hydrangea. Proc Calif Acad Sci 29:147-256   

11. Morell MK, Peakall R, Appels R, Preston LR, Lloyd HL (1995) DNA profiling techniques for plant variety identification. Aust J Exp Agric 35:807-819. doi:10.1071/EA9950807  

12. Ohba H (2001) Hydrangea Gronov. ex L. In K Iwatsuki, DE Boufford, H Ohba, eds, Flora of Japan, 2b. Kodansha, Tokyo, Japan pp 84-94  

13. Reed SM, Rinehart TA (2007) SSR marker analysis of genetic relationships within Hydrangea macrophylla. J Am Soc Hortic Sci 132:341-351  

14. RHS Colour Chart (2015) Royal Horticultural Society (RHS) Color Chart, 6th ed. RHS, London, UK  

15. Rinehart TA, Scheffler BE, Reed SM (2006) Genetic diversity estimates for the genus Hydrangea and development of a molecular key based on SSR. J Am Soc Hortic Sci 131:787-797   

16. Ryan PD, Harper DAT, Whalley JS (1995) PALSTAT, Statistics for Palaeontologists. Chapman& Hall, London, UK  

17. van Gelderen CJ, van Gelderen DM (2004) Encyclopedia of Hydrangeas. Timber Press, Portland, Oregon, USA  

18. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res18:6531-6535. doi:10.1093/nar/18.22.6531  

19. Wilson EH (1923) The hortensias: Hydrangea macrophylla DC. and Hydrangea serrata DC. J Arnold Arbor 4:233-246   

20. Wolfe AD, Xiang Q-Y, Hephart SR (1998) Assessing hybridization in natural populations of Penstemon (Scrophulariaceae) using hyper variable inter simple sequence repeat (ISSR) bands. Mol Ecol 7:1107-1125. doi:10.1046/j.1365-294x.1998.00425.x  

21. Zonneveld BJM (2004) Genome size in Hydrangea, In CJ van Gelderen,DM van Gelderen, eds, Encyclopedia of Hydrangeas. Timber Press, Portland, Oregon, USA. pp 245-251